AmpliScribe™ T7 High Yield Transcription Kit

Produce high yields of high quality RNA quickly and easily.

  • Synthesize long and short RNA with one kit
  • Produce >20 times more RNA than conventional or “homemade” in vitro transcription kits
Name SKU Size Availability Vendor Price Order
AmpliScribe™ T7 High Yield Transcription Kit AS3107 50 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Applications

  • High-yield synthesis of RNA from DNA cloned downstream from a T7 RNA polymerase promoter, for microinjection, RNA interference, or antisense experiments.
  • Structural and functional studies of splicing, processing, heterogeneous nuclear RNAs, tRNAs, viral RNAs, and ribozymes.1
  • Production of fluorescent2-, biotinylated3-, or digoxigenin4– labeled RNA.

The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to use high concentrations of NTPs, that can be inhibitory to other in vitro transcription systems. AmpliScribe High Yield Transcription reactions can produce >20-fold more full-length RNA transcript than conventional in vitro transcription reactions. For example, the AmpliScribe T7 Kit yields approximately 5-7.5 mg/ml of 1.4-kb full-length control RNA in 30-120 minutes, respectively, using 1 µg of template DNA (Fig. 1).

Features

  • High yield. Produce microgram amounts of RNA from all templates—synthesize both long (Fig. 2) and short RNA using only one kit.5
  • Sensitive. Produce microgram amounts of RNA from as little as 1 ng of template.
  • Scaleable. Increase the reaction size to produce milligram amounts of RNA.
  • Flexible. Substitute a labeled nucleotide for one of the supplied NTP solutions.
  • Convenient. Rnase inhibitor included.

Note: Because of the very high concentration of NTPs used in AmpliScribe reactions, these kits are not recommended for preparing high specific-activity radiolabeled RNA probes.

References

  1. Johnson, M. (1995) Epicentre Forum 2(5), 4.
  2. DeLong, E.F. et al. (1999) App. And Environ. Microbiol. 65, 5554.
  3. Kaplan, E. et al. (1996) Epicentre Forum 3(2), 1.
  4. Hoff man, L.M. and Johnson, M.G. (1994) BioTechniques 17, 372.
  5. Schanke, J. (2000) Epicentre Forum 7(2), 6.
  6. Molecular Cloning – A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 – 7.34. J. Sambrook and D. Russell.

Resources

Protocol:

SDS:

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