AccuStart Genotyping ToughMix

Features

Genotyping ToughMix is a 1-tube qPCR SuperMix reagent compatible with all dual-label (hydrolysis) probe chemistries for both fast and conventional PCR cycling protocols or instruments. This proprietary formulation has been rigorously optimized to destabilize single base-pair mismatches to ensure precise allelic discrimination and cluster separation with SNP detection assays. The reagent is provided as a 2x concentrated ready-to-use reaction cocktail that contains all required reaction components, except primers, probe(s), and DNA template. Inert AccuVue plate loading dye helps to minimize pipette error and provides visual confirmation of thorough mixing.

Features & Benefits

• Optimized buffer chemistry destabilizes single base-pair mismatch probes, providing superior allelic discrimination and improved cluster separation for critical, single-nucleotide polymorphism (SNP) detection assays
• Sensitive, precise detection with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
• ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
• Easy-to-use 2x concentrated SuperMix with AccuVue plate loading dye and pre-blended passive reference dye simplifies reaction setup
• Supports efficient vortex mixing with proprietary anti-foaming technology

Genotyping ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Contents

Single-tube, 2X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP.
• AccuStart II Taq DNA Polymerase.
• Inert AccuVue dye.
• Proprietary enzyme stabilizers and performance-enhancing additives.
• Titrated reference dye (if applicable).

Storage & Handling

• Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
• After thawing, mix thoroughly before using. Repeated freezing and thawing does not affect PCR performance.
• For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.