AccuGreen™ High Sensitivity dsDNA Quantitation Kit

A fluorescent dsDNA quantitation kit with a linear range of 0.1-100 ng DNA. Designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher Scientific.

Name SKU Size Availability Vendor Price Order

AccuGreen™ High Sensitivity dsDNA Quantitation Kit, trial size.

 31066-T 100 assays Generally 1-2 weeks from receipt of order Biotium Log in for pricing

AccuGreen™ High Sensitivity dsDNA Quantitation Kit

 31066 500 assays Generally 1-2 weeks from receipt of order Biotium Log in for pricing

Photos

Features

The AccuGreen™ High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples. The kit provides a linear detection range from 0.1 – 100 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific. The AccuGreen™ High Sensitivity assay is also available without a DNA standard (31068), for those that wish to use their own standard.

Features

  • Designed specifically for Thermo’s Qubit® fluorometer
  • Linear range: 0.1-100 ng dsDNA
  • Sample concentration range: 5 pg/uL to 100 ng/uL
  • Green fluorescence emission
  • Replaces Qubit® dsDNA HS Assay Kit for cost savings

Kit Components

  • AccuGreen™ Dye, 200X
  • DNA Quantitation Buffer, 20X Concentrate*
  • AccuGreen™ Standard 1 (0 ng/uL)
  • AccuGreen™ Standard 2 (10 ng/uL)
    *Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

AccuGreen™ for the Qubit® fluorometer
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific.

The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.

Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

Resources

Protocol:

SDS:

DNA & RNA Quantitation Flyer:

Molecular Biology Brochure:

Earth Friendly Products Flyer:

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