AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit

  • Produce micrograms of biotin-RNA or biotin-aRNA
  • Save time with 30 minute high-yield protocol, or extend the incubation to increase yield even further
Name SKU Size Availability Vendor Price Order
AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit ASB71110 10 Rxns Generally 1-2 weeks from receipt of order LGC Biosearch Technologies - Lucigen (Epicentre) Log in for pricing

Applications

  • Production of nonradioactive RNA probes for any purpose, including microarray (transcription profiling) studies, in situ hybridization, and blotting experiments.

The AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit enables high-yield synthesis of biotin-RNA or biotin-antisense RNA (aRNA, also called cRNA). The kit incorporates Biotin-16-UTP directly into the RNA transcript. High yields of biotin-RNA are produced from as little as 50 ng of input DNA template.

Features

  • Exceptionally high yields. The kit uses Epicentre’s AmpliScribe T7-Flash rapid, high-yield in vitro transcription technology to generate the highest possible yield of biotin-RNA.
  • Optimized. In vitro transcription reaction conditions maximize yield of uniformly labeled biotin-RNA for high signal intensity.
  • Convenient. Rnase Inhibitor included.
Incubation Time (minutes)
30 60 (1 hour) 120 (2 hours) 480 (4 hours)
Template DNA 50 ng 5 – 10 µg 15 – 25 µg 30 – 40 µg 60 – 70 µg
100 ng 15 – 20 µg 35 – 45 µg 70 – 80 µg 130 – 140 µg
500 ng 60 – 70 µg 140 – 150 µg > 180 µg > 180 µg
1.0 µg 125 – 135 µg > 180 µg > 180 µg > 180 µg
Table 1. Yield of Biotin-RNA (in mg) from varying amounts of control template DNA from a standard 37°C, 20 ml AmpliScribe T7-Flash Biotin-RNA Transcription Kit reaction over time. Results may vary depending on the template used.

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