Literature Digest Viral Integrity with PMA & PMAxx™
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PMA (Propidium Monoazide) |  |
PMAxx™ Dye, 20 mM in H2O |
PMA & PMAxx™ combined with qPCR is a well-published method for sensitive, accurate, and rapid detection of intact viral capsids. In this digest, we have selected three publications with summaries and takeaways on the use of this method for evaluating viral integrity.
A systematic and comprehensive literature review was conducted to establish protocols and considerations for viral integrity qPCR methods. The study focused on publications using the azo dyes PMA, PMAxx™, PEMAX, and EMA for capsid integrity monitoring. The search was performed in relevant databases including Pubmed, Scopus, Ovid, Medline, and Web of Knowledge since the first introduction of the azo dye pretreatment in 2003.
