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Denovix | Comparing Hemocytometers and Automated Cell Counters
Written by Ben Capozzoli, DeNovix Application Scientist Since the invention of the hemocytometer counting chamber in 1874, biologists have been quantifying cells dispensed into the chamber of the slide through manual inspection under a microscope. These quantifications rely on knowing the exact volume in which you are counting. The following equation is used to translate […]
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Denovix | Is Erythrosin B a Better Choice than Trypan Blue for Cell Counting?
Written by Famatta Perry, PhD Cell counting is important for reproducible and accurate documentation of cell concentrations and viability, which can be used in a series of research, medical and biotechnological applications. Despite numerous documented issues with and limitations of Trypan Blue, it is still widely used in many research labs. This article aims to […]
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Denovix | Flow Cytometry and Image-Based Cell Counting: Complementary Technologies to Achieve Accurate Cell Quantifications and Characterizations
Written by Ben Capozzoli, DeNovix Application Scientist Understanding the number and characteristics of a given cell population is paramount to many cell/molecular biology studies. Many fields, like immunology, rely heavily upon the ability to quantify the number of cells in a population and the various characteristics those cells possess. Hemocytometers: A Traditional Cell Counting Method […]
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Denovix | How to Determine Cell Density of Microbial Cultures with OD600 Measurements and Factors
Written by Rachel Kennel, DeNovix Application Scientist The measurement of optical density (OD) at 600 nm is a common spectrophotometric method to determine the density of a bacterial or yeast culture, commonly during phases of bacterial or yeast growth. An OD600 measurement is a quick and convenient way to determine density for cells, which are […]
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DeNovix | DS-11 Series Compared with the Thermo Fisher NanoDrop™ One Spectrophotometer
The DeNovix DS-11 Series Spectrophotometer / Fluorometer and the Thermo Fisher NanoDrop™ One spectrophotometer are both used to perform fast quantification of nucleic acids and proteins. The DeNovix DS-11 Series provides superior limits of detection, the widest dynamic range, an easy-to-use interface and powerful networking features, which surpass the capabilities of the Thermo Fisher NanoDrop™ […]
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Denovix | Sample QC: Absorbance or Fluorescence Quantification?
Absorbance and Fluorescence quantification are unique but complementary methods for assessing concentration and quality of biological samples. Selecting the appropriate quantification methods for biological samples is an important consideration in any quality control process. Download our infographic featuring comparisons of the key features of absorbance and fluorescence quantification and information on when each method should […]
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Denovix | Fluorescence Measurement Best Practices
Fluorometric quantitation assays provide a quick and easy way to measure DNA concentrations and are a routine part of analyzing nucleic acids and proteins. However, inefficient workflows may result in errors down the line. Whether you’re curious about optimizing sample prep or LED selection, check out this resource on the best practices for fluorescence measurement.
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Denovix | The Working Principle of a Fluorometer
Fluorometers are indispensable tools used for precise quantitation of various biological molecules, such as nucleic acids and proteins, in microliter (μL) samples. These instruments play a crucial role in a wide range of biochemical fields, including life sciences, environmental monitoring, pharmaceutical quality control, and bioengineering applications. By providing high-sensitivity measurements, fluorometers enable the quantification of […]
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Denovix | Counting and Quality Control of Isolated Nuclei
As samples are prepared for single cell sequencing workflows, it’s important to perform quality control checks. The CellDrop Automated Cell Counter offers a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample. Download our new infographic below, to explore recommended methods for optimizing nuclei counts before downstream sequencing […]
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