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Biotium | Tech Tip: Isolation and Staining of Extracellular Vesicles
Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, EVs and their cargo are used as diagnostic biomarkers for cancer and other diseases. However, the isolation and detection of EVs can be extremely challenging due to their […]
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Biotium | Protocols and Tips for Success with the EvaEZ™ Fluorometric PAAK
EvaEZ™ is a fluorescence-based assay that allows you to quantitate DNA polymerase activity without using radioisotopes. While the assay is simple to use, there are many factors that can affect the quality of your data. In this article, we’ve outlined our advice for getting the best results using our EvaEZ™ Assay with your enzyme. EvaEZ™ […]
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Biotium | Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Cross-talk and specificity in multi-color imaging experiments Cross-talk between fluorescence channels is an important concern for multi-color imaging experiments. The detection of fluorescence from one dye in multiple detection channels can result in artifactual co-localization of signals. Cross-talk usually occurs because of the overlap of dye excitation and emission spectra, and can be managed by […]
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Biotium | Tech Tip: Staining Membranes in Fixed Cells (CytoLiner™ Protocol)
CytoLiner™ Fixed Cell Membrane Stains are unique lipophilic fluorescent dyes that provide selective and uniform plasma membrane staining in formaldehyde-fixed cells, ensuring consistent results. Unlike other lipophilic dyes, such as DiO, Dil, Vybrant® dyes, or CellMask™ Deep Red, CytoLiner™ Dyes have optimized chemical structures that balance solubility with lipophilicity, which permits more homogenous and reliable […]
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Biotium | Five Steps to Successful Cell Membrane Staining & Imaging
Membrane and cell surface stains are useful for visualizing cell borders and morphology in multicolor staining of live or fixed cells. Biotium offers several options for cell surface imaging, including the original CellBrite® Cytoplasmic Membrane Stains, which are specialized formulations of classic lipophilic carbocyanine dyes for membrane labeling. Since their release, Biotium scientists have designed […]
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Biotium | Guide for Selecting the Right Cell Surface Stain
Membrane staining is useful in defining cell boundaries in multicolour imaging studies, but finding dyes compatible with the various viability, fixation, and experimental staining conditions dictated by a particular workflow or application can be challenging. In order to solve this issue, Biotium has created a versatile selection of highly fluorescent and photostable stains to visualize […]
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Biotium | GelRed® and GelGreen® Tech Tip
Eight things to keep in mind for optimal gel staining with GelRed® and GelGreen® GelRed® ORDER HERE GelGreen® ORDER HERE The most common use of GelRed® and GelGreen® nucleic acid stains is in pre-cast agarose gels, where the dyes are added to molten agarose during gel preparation. However, due to their larger size designed to […]
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Biotium | Cheetah™ FLEX HotStart Taq DNA Polymerase
Introducing Cheetah™ FLEX and Cheetah™ PEA-PLEX HotStart Taq DNA Polymerases for qPCR, PEA, and NGS-Based PEA Workflows ORDER HERE Cheetah™ Taq requires only 2 minutes of heat activation for full recovery of activity. Comparison of hot-start recovery of polymerase activity for Cheetah™ Taq and AmpliTaq Gold® following incubation at 95°C in 50 mM pH 8.0 […]
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Biotium | Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase
Introducing Cheetah™ FLEX and Cheetah™ PEA-PLEX HotStart Taq DNA Polymerases for qPCR, PEA, and NGS-Based PEA Workflows ORDER HERE Cheetah™ Taq requires only 2 minutes of heat activation for full recovery of activity. Comparison of hot-start recovery of polymerase activity for Cheetah™ Taq and AmpliTaq Gold® following incubation at 95°C in 50 mM pH 8.0 […]
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Biotium | RNaseReveal™ Activity Assay Kit
Discover Biotium’s new RNaseReveal™ Activity Assay Kit—ultrasensitive RNase detection with a fast, easy protocol to streamline your workflow. Fremont, CA – April 25, 2025 – Biotium, a leading developer of fluorescent reagents for life science research, announces the launch of the RNaseReveal™ Activity Assay Kit, with unparalleled sensitivity to detect RNase contamination at levels as low as 0.08 […]
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