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Biotium | PMAxx™ q-PCR: an effective tool for detection of seed-borne pathogens
PMAxx™ Dye, 20 mM in H2O ORDER HERE Seed health is important for good yields and maintaining desired plant traits, as well as for minimizing the spread and dispersal of plant pathogens. The certification of pathogen-free seeds is an effective strategy to prevent and manage disease. This approach, however, is highly dependent on a reliable […]
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Biotium | Literature Digest: Monitoring Viable Bacteria in the Environment
PMA (Propidium Monoazide) ORDER HERE PMA-Lite™ 2.0 LED Photolysis Device ORDER HERE Donwload Literature Digest.
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Biotium | Bring Out Your Dead: v-PCR for Microbial Population and Diversity Studies
Understanding microbes and their ecology is crucial for modern medicine and functioning society. Microbes cause disease, but are also vital components of a healthy gut and skin microbiome. Microbes fix nitrogen for plants, produce oxygen in marine environments, facilitate rusting and infrastructure breakdown, and come with us to space. By better understanding microbial diversity and […]
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Biotium | Switching from agar plates to v-PCR for bacteria viability testing – What you need to know
The problem with plating Counting the number of living bacteria in a sample is a common enough task: maybe you are comparing bacteria populations in different rivers, or perhaps you want to know whether your disinfection method works. Your first thought is probably to prepare serial dilutions of a liquid culture, plate them out on […]
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Biotium | Tech Tip: Imaging Bacteria Using Agarose Pads
Hoechst ORDER HERE Viability/Cytotoxicity Assay Kit for Bacteria Live & Dead Cells ORDER HERE Tech tip: Imaging bacteria using the agarose pad technique Bacteria can be more difficult to image than other cell types because they are small, usually non-adherent, and are often motile. Motile live bacteria are more difficult to image than dead […]
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Biotium | Tips & Best Practices for Working with RNA
Considerations for Doing RNA Work Working with RNA can be challenging and intimidating compared to working with DNA. While DNA is known to be quite stable and hardy (geneticists have been sequencing genomes that are thousands of years old), the chemical structure of RNA makes it prone to hydrolysis and, therefore, much less stable. And […]
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Biotium | Tech Tip: Isolation and Staining of Extracellular Vesicles
Extracellular vesicles (EVs) are small, membrane-bound particles secreted from cells and thought to function as cellular messengers, carrying cargo from one cell to another. In biomedical research, EVs and their cargo are used as diagnostic biomarkers for cancer and other diseases. However, the isolation and detection of EVs can be extremely challenging due to their […]
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Biotium | Protocols and Tips for Success with the EvaEZ™ Fluorometric PAAK
EvaEZ™ is a fluorescence-based assay that allows you to quantitate DNA polymerase activity without using radioisotopes. While the assay is simple to use, there are many factors that can affect the quality of your data. In this article, we’ve outlined our advice for getting the best results using our EvaEZ™ Assay with your enzyme. EvaEZ™ […]
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Biotium | Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Cross-talk and specificity in multi-color imaging experiments Cross-talk between fluorescence channels is an important concern for multi-color imaging experiments. The detection of fluorescence from one dye in multiple detection channels can result in artifactual co-localization of signals. Cross-talk usually occurs because of the overlap of dye excitation and emission spectra, and can be managed by […]
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Biotium | Tech Tip: Staining Membranes in Fixed Cells (CytoLiner™ Protocol)
CytoLiner™ Fixed Cell Membrane Stains are unique lipophilic fluorescent dyes that provide selective and uniform plasma membrane staining in formaldehyde-fixed cells, ensuring consistent results. Unlike other lipophilic dyes, such as DiO, Dil, Vybrant® dyes, or CellMask™ Deep Red, CytoLiner™ Dyes have optimized chemical structures that balance solubility with lipophilicity, which permits more homogenous and reliable […]
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