Validated Antibodies for Detection of EV Markers by Flow Cytometry
The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. While antibodies targeting these proteins are available by commercial suppliers, few are validated or perform well for detection of EVs or exosomes. The antibodies and dye labels of ExoBrite™ Flow Antibody Conjugates were carefully selected and validated for robust detection of isolated EVs. In addition, the antibodies are provided in a proprietary buffer formulation for reduced antibody aggregation and brighter EV staining for optimal accuracy and signal-to-noise.
ExoBrite™ Isotype Control Flow Antibody, which have been found to not react with any target in human cells and have the same isotype as the tetraspanin antibodies, are also available as a negative control.
Advantages of ExoBrite™ Flow Antibodies:
- NEW: Now with updated buffer formulation for improved signal-to-noise
- Developed for detection of EV markers CD9, CD63, and CD81 by flow cytometry
- Validated for purified or bead-bound EVs
- Bright signal and low background
- ExoBrite™ Isotype Control Flow Antibody available
- Colours available for Pacific Blue™, FITC, PE, and APC channels
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ExoBrite™ CD9 Flow Antibody |
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ExoBrite™ CD9 (Mouse) Flow Antibody |
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ExoBrite™ CD63 Flow Antibody |
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ExoBrite™ CD63 (Mouse) Flow Antibody |
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ExoBrite™ CD81 Flow Antibody |
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ExoBrite™ CD81 (Mouse/Rat) Flow Antibody |
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ExoBrite™ IgG1 Isotype Control Flow Antibody |