Membrane & Cell Surface Stains Brochure –
Brochure and guide on our various membrane stains, binding mechanisms, and recommendations for different applications and workflows.
Biotium offers a variety of bright and photostable stains to visualize cell boundaries and morphology in multi-color staining experiments. This selection includes traditional and novel stains designed for livecell imaging, convenient labeling of fixed cells, or the staining of live cells that will be subsequently fixed and immunostained.
CellBrite® Fix and MemBrite® Fix stains are novel membrane stains designed to label live cells for downstream immunofluorescence (IF) microscopy. The stains withstand both fixation and permeabilization and are available in a wide selection of colors.
CellBrite® Steady Membrane Staining Kits allow fluorescence imaging of live cells for up to several days in culture. The CellBrite® Steady Dyes are unique fluorescent membrane probes that equilibrate between the cell surface and intracellular compartments, allowing cells to retain cell surface staining over time. Our unique CellBrite® Steady Enhancer can optionally be used to reduce intracellular fluorescence for selective imaging of cell boundaries.
Our original CellBrite® Cytoplasmic Membrane Dyes are ready-touse staining solutions of well-characterized lipophilic carbocyanine dyes for non-toxic and stable labeling of membranes in live or fixed mammalian cells. We also offer novel near-IR dye lipophilic dye options, which are ideal for non-invasive small-animal imaging studies of cell migration and cell homing due to their long emission wavelengths. If desired, cells can be fixed with formaldehyde before or after staining.
We also offer the lectins WGA and Con A conjugated to our highperformance CF® Dyes. Lectins label glycoproteins on the surface of live or fixed cells. In permeabilized cells, these stains may also label secretory and endocytic compartments. Lectin staining may be celltype dependent in mammalian cells. Some fluorescent lectins are useful for bacterial Gram stains and yeast cell surface stains.
